High-throughput metabarcoding methods have become a routine analysis tool in biodiversity research and ecology in general. This course will cover the principles of metabarcoding workflow, from the laboratory analyses to bioinformatic analyses of the high-throughput sequencing data.
Laboratory work includes steps for sample preparation, DNA extraction, PCR and sequencing library preparation. The practical sessions are supplemented with theoretical lectures.
Hands-on metabarcoding data set analyses will mostly follow PipeCraft 2 pipeline (user-friendly graphical user interface software for non-bioinformaticians) but basics of command-line interface and usage of high-performance clusters will be also covered.
- Prof. Leho Tedersoo, University of Tartu
- Prof. Urmas Kõljalg, University of Tartu
- Dr. Sten Anslan, University of Tartu Dr. Vladimir Mikryukov, University of Tartu
- Dr. Kessy Abarenkov, University of Tartu
- Dr. Niloufar Hagh-Doust, University of Tartu
- Dr. Heidi Tamm, University of Tartu
- Ali Hakimzadeh, University of Tartu
Dr. Niloufar Hagh-Doust, Research fellow at University of Tartu
1. Understanding the main concepts, potentials and limitations of metabarcoding techniques.
2. Knowledge of the laboratory procedures necessary for metabarcoding.
3. Ability to set up the working environment for bioinformatic analysis.
4. Knowledge of key bioinformatic procedures for metabarcoding sequence data analyses.
5. Understanding of the full cycle of data management and publishing.
EUR 500: Students are responsible for their travel, accommodation and travel insurance (visa arrangements if needed) from their home country to Tartu and back to their home country. It is recommended to visit the Tartu Welcome Centre website and Student Hostel website to find accommodation opportunities. It is possible to take a bus from Tallinn Airport to Tartu Coach Station (180km). Ticket information on Tpilet.
For further information, please click the "LINK TO ORIGINAL" button below.